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L'amélioration des performances de l'huître plate européenne Ostrea edulis par la sélection génétique ArchiMer
Bedier, Edouard; Baud, Jean-pierre; Cochennec, Nathalie; Cornette, Florence; Gerard, Andre; Goyard, Emmanuel; Joly, Jean-pierre; Kopp, Joel; Langlade, Aime; Le Coguic, Yvette; Mazurie, Joseph; Nourry, Max.
The European flat oyster Ostrea edulis has been considered as the traditional French oyster for a very long time and has now nearly disappeared from our farms, as a consequence of the two successive epizootic of the Marteilia refringens parasite and Bonamia ostreae. The actual quasi monoculture of Crassostrea gigas has made necessary the search for a diversification in oyster culture, including the search for a new start of the indigenous flat oyster culture. The Ifremer has been conducting parallel works since 1985 in order to better understand the biology of the parasite as well as to try and select different lines of flat oyster resistant to Bonamia ostreae.
Tipo: Text Palavras-chave: Bonamia ostrea; Huître plate; Sélection Génétique; Ostrea edulis.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3274.pdf
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Etude de faisabilité d’opérations de ‘repeuplement orienté’ dans deux sites français de captage de l’huître creuse (Crassostrea gigas) ArchiMer
Bernard, Ismael; Lapegue, Sylvie; Dumas, Franck; Talarmain, Eric; Cornette, Florence; Degremont, Lionel; Chavanne, Herve; Pouvreau, Stephane; Auby, Isabelle; Maurer, Daniele; Plus, Martin; Le Moine, Olivier; Boudry, Pierre.
La forte augmentation des mortalités de juvéniles d’huître creuse depuis 2008 a relancé la problématique de l’approvisionnement en naissains de l’ostréiculture française. Dans ce contexte, cette étude a pour objectif d’étudier la faisabilité d’opérations de repeuplement en huîtres génétiquement plus résistantes, afin d’améliorer les performances de survie du naissain capté dans le milieu naturel. Cette étude s’est ainsi focalisée sur les deux bassins naisseurs historiques, Marennes-Oléron et Arcachon. Nous avons donc cherché à établir (1) une hiérarchie des bancs en fonction de l’expulsion de larves d’huître dans le bassin d’Arcachon, (2) une cartographie des liens en termes de connectivité entre les bancs des pertuis charentais et (3) la faisabilité du...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19410/17013.pdf
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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Mise en évidence expérimentale de la variance du succès reproducteur chez l'huître creuse Crassostrea gigas (Thunberg) ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Kotoulas, G.; Bonhomme, Francois.
Oysters, like many other marine species, are characterized by very high fertilities. Natural or hatchery populations, often present very low Ne/N ratios (Hedgecock, 1994). It can be explained by very high variances of their breeding success (Li and Hedgecock, 1998), the effective population size being estimated through the temporal variance of the allelic frequencies in natural populations (Hedgecock et al., 1992) or, more directly, through the estimated number of founders in hatchery populations (Launey, 1998). This document is a presentation of the results of a number of experiments, realised in the scope of the European program "GENEPHYS" (FAIR 95-421), and using microsatellite markers to analyse the parental contributions in the flat oyster Crassostrea...
Tipo: Text Palavras-chave: Microsatellite; Succès reproducteur; Contributions parentales; Génétique; Crassostrea gigas; Huîtres.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3454.pdf
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Utilisation des marqueurs microsatellites pour l'étude des contributions parentales chez l'huître creuse Crassostrea gigas ArchiMer
Boudry, Pierre; Collet, Bertrand; Kotoulas, G.; Hervouet, Veronique; Cornette, Florence; Bonhomme, Francois; Gerard, Andre.
Numerous aquacultural species show very high fertilities. As a consequence, it is possible to produce, in hatcheries, a large amount of sibs from a small amount of parents. The sibs' genetic variability is, of course, directly linked to the number of parents involved, but it may be largely lowered by imbalanced parental contributions. Polymorphic genetic markers are very handy tools in the study of these phenomena in cultivated populations (Harris et al. 1991; Herbinger et al., 1996a; Garcia de Leon et al. 1998) or in natural populations (Ferguson et al, 1995; Kellog et al., 1995; Colbourne et al., 1996; Herbinger et al., 1996b). This document is a presentation of the results of a number of experiments, realised in the scope of the European program...
Tipo: Text Palavras-chave: Microsatellites; Contributions parentales; Crassostrea gigas; Huîtres.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3452.pdf
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Ressources génétiques chez les huîtres creuses : conservatoire de souche et marqueurs moléculaires ArchiMer
Boudry, Pierre; Heurtebise, Serge; Collet, Bertrand; Cornette, Florence; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
The cupped oyster strain bank was created at Ifremer at La Tremblade in 1992. It was funded by the Poitou-Charentes regional council. Its goals were: 1. to study the intra- and interspecific genetic differentiation within the genus Crassostrea. 2. to study acclimatization potential of foreign oysters in French waters in order to identify species that could replace Crassostrea gigas in case of a major disease outbreak in C. gigas or in case of a need or desire to diversify shellfish production. 3. to study the possibility of interspecific hybridisation in the genus Crassostrea and hybrid performance.
Tipo: Text Palavras-chave: Saccostrea commercialis; Crassostrea rhisophorae; Crassostrea gasar; Crassostrea sikamea; Crassostrea virginica; Crassostrea rivularis; Crassotea angulata; Crassostrea gigas; Génétique.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3242.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996 ArchiMer
Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf
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A la recherche de l'huître portuguaise: identification de marqueurs moléculaires discriminant Crassostrea angulata et Crassostrea gigas ArchiMer
Cornette, Florence.
L'étude historique de l'ostréiculture française monte que cette activité est caractérisée par des périodes de prospérités et de pénurie. Des épizooties ont été à l'origine de ces alternances. A chacune de ces période, on a pu constater des changements d'espèces : Ostrea edulis fut remplacée par Crassostrea angulata, de même celle-ci fut remplacée par Crassostrea gigas dans les années 1970. L'étude de la bibliographie montre que les techniques utilisées pour distinguer des espèces Crassostrea angulata et Crassostrea gigas sont insuffisantes. L'évolution des technologies en matière d'analyses génétiques ouvrent de nouvelles perspectives. La recherche de marqueurs moléculaires réalisée sur Crassostrea angulata et Crassostrea gigas, par amplification par PCR...
Tipo: Text Palavras-chave: Crassostrea angulata; Crassostrea gigas; Marqueurs moléculaires; ADN mitochondrial; PCR; Enzymes de restrictions; Haplotypes.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00049/15979/13421.pdf
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ReMoNor : Résultats 2000 ArchiMer
Cornette, Florence; Blin, J.l.; Pacary, S; Pien, S; Le Gagneur, Eric; Kopp, Joel; Richard, O.
At the end of its third year, the regional network for monitoring REMONOR, confirms the particularities of oyster from Normandy in matter of growth and quality. Results from 2 000 are globally very close of those from 1998. Mortality of adults reached 14% and juvenile 6% which represent a low death rate. For this year the most blow area is the Baie des Veys with 31% death rate at Géfosse. Observed growth this year had been weaker than the past two years despite the spring and automnal growth. At spring, the higher recorded growth rate on the West coast with 0.42% (regional average : 0.29%). This seasonal trend seems more and more obvious every year. Regarding the classification of the special oysters, despite the change of the standard, the Baie des Veys...
Tipo: Text Palavras-chave: REMORA; Quality; Growth; Mortality; Normandy; REMONOR; Crassostrea gigas; REMORA; Qualité; Croissance; Mortalité; Basse Normandie; REMONOR; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/rapport-2251.pdf
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ReMoNor : Résultats 2001 ArchiMer
Cornette, Florence; Blin, J.l.; Pien, S; Simonne, Charlotte; Kopp, Joel; Richard, O.
The regional network REMONOR showed 2001's characteristics of growth and quality of oysters (Crassostrea gigas) in the three rearing areas of Normandy. Compared to year 2000, two additional stations along the East Coast have been settled. The summer adults mortalities have been registered between 0 % and 25 %. The average for all the rearing areas reached 6 %. Highest rates have been noticed in the Baie des Veys and the East Coast. The juvenile's mortality occurred during the same season, in summer. This year 2001 is included in lower growth years in all Normandy. This year, the West Coast growth rate appeared to be quit unusual. For the first time growth rates were in the average in the northern area that usually shows very bad performance. We can...
Tipo: Text Palavras-chave: REMORA; Quality; Growth; Mortality; Normandy; REMONOR; Crassostrea gigas; REMORA; Qualité; Croissance; Mortalité; Basse Normandie; REMONOR; Crassostrea gigas.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/rapport-2249.pdf
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Niveau de ploïdie des huîtres des bassins de captage de Marennes-Oléron et Arcachon ArchiMer
Cornette, Florence; Grouhel, Stephanie; Sharbel, Tim; Boudry, Pierre; Goulletquer, Philippe; Lapegue, Sylvie.
La mise en place du réseau « biovigilance » résulte des recommandations formulées dans le cadre de l’expertise indépendante demandée par le Comité Scientifique du Ministère de l’Agriculture et de la Pêche concernant « l’effet d’un flux éventuel d’huîtres tétraploïdes dans les zones conchylicoles ». Il avait été alors préconisé de réaliser « une biovigilance légère, avec mesure régulière du taux d’huîtres tétraploïdes dans les bassins conchylicoles ». Le sous-projet « Réseau biovigilance » s’inscrit dans le projet Ifremer « Typologie et surveillance des systèmes de productions aquacoles » du programme de recherches «Durabilité des systèmes de production » du thème « Surveillance et optimisation des productions aquacoles ». Ce réseau a pour objectif d’être...
Tipo: Text Palavras-chave: Reproduction; Captage; Arcachon; Marennes Oléron; Génétique; Crassostrea gigas; Ploïdie; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00043/15445/12815.pdf
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Variabilité génétique des stocks d'huîtres dans les Pertuis charentais ArchiMer
Cornette, Florence; Lapegue, Sylvie.
Afin de fiabiliser l'approvisionnement en juvéniles de bivalves dans les Pertuis charentais, une information importante à prendre en compte est l'origine des naissains captés. Ainsi, afin de tenter de déterminer la provenance du naissain capté dans chaque zone des Pertuis charentais, l'objectif de l'action Vargenet « Variabilité génétique des stocks d'huîtres dans les Pertuis charentais» est de déterminer si l'outil génétique peut être envisagé en complément des données de suivi de stocks et de modélisation. Pour cela, il est nécessaire de savoir si les stocks sauvages présents dans ces zones peuvent être différenciés génétiquement. Si c'est le cas, les cohortes de naissain captées pourraient alors être assignées à un stock parental, ce qui permettrait...
Tipo: Text Palavras-chave: Génétique; Variabilité génétique; Naissain; Stocks sauvages; Huître creuse; Crassostrea gigas; Pertuis Charentais; Charente-Maritime.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00055/16661/14154.pdf
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REseau MOllusques des Rendements Aquacoles huitre creuse (REMORA) ; Résultats des stations nationales. Année 2000 ArchiMer
Fleury, Pierre-gildas; Cornette, Florence; Claude, Serge; Palvadeau, Hubert; Robert, Stéphane; D'Amico, Florence; Le Gall, Patrik; Vercelli, Catherine.
The monitoring network IFREMER /REMORA is assessing, on a yearly basis, mortality, growth and quality criteria of two oyster batches ("juveniles" = one-year old ; and "adults" = two-years old), distributed among various stations of the main French oyster areas. Samplings are carried out in March, June, September and December. This network is a tool for trend estimations (geographic and chronological, on the national scale) ; it must not be considered as an accurate estimation of professional oyster ongrowings. With the exception of Spring mortalities due to poor initial quality of adult oysters and to difficulties in the counting of juveniles, the year 2000 appeared to be a year of low mortality. 2000 is a year of poor growth (61.5 g in average weight in...
Tipo: Text Palavras-chave: Polydora; Quality; Growth; Mortality; Crassostrea gigas; Cupped (gigas) oyster; France; REMORA; Polydora; Qualité; Croissance; Mortality; Crassostrea gigas; Huître creuse; France; REMORA.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/rapport-1635.pdf
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REseau MOllusques des Rendements Aquacoles huitre creuse (REMORA) ; résultats des stations NATIONALES ; Année 2001 ArchiMer
Fleury, Pierre-gildas; Cornette, Florence; Claude, Serge; Palvadeau, Hubert; Robert, Stephane; D'Amico, Florence; Le Gall, Patrik; Vercelli, Catherine; Pien, S.
The monitoring network IFREMER /REMORA is assessing, on a yearly basis, mortality, growth and quality criteria of two oyster batches ("juveniles" = one-year old ; and "adults" = two-years old), distributed among various stations of the main French oyster areas. Samplings are carried out in March, June, September and December. This network is a tool for trend estimations (geographic and chronological, on the national scale) ; it must not be considered as an accurate estimation of professional oyster ongrowings. In 2001, mortality mainly occured in Summer on "adults" in baie des Veys (Normandy) and on juveniles in Cancale and Pénerf (Brittany) and in Fouras and Marennes (Poitou-Charentes). With an average individual weigh of 59 g in December, 2001 has been a...
Tipo: Text Palavras-chave: Polydora; Quality; Growth; Mortality; Crassostrea gigas; Cupped (gigas) oyster; France; REMORA; Polydora; Qualité; Croissance; Mortalité; Crassostrea gigas; Huître creuse; France; REMORA.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/rapport-2438.pdf
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REseau MOllusques des Rendements Aquacoles huitre creuse (REMORA) ; Résultats des stations nationales. Année 1999 ArchiMer
Fleury, Pierre-gildas; Cornette, Florence; Claude, Serge; Palvadeau, Hubert; Robert, Stéphane; Vercelli, Catherine; Chabirand, Jean-michel; D'Amico, Florence.
The monitoring network IFREMER /REMORA is assessing, on a yearly basis, mortality, growth and quality criteria of two oyster batches ("juveniles" = one-year old ; and "adults" = two-years old), distributed among various stations of the main French oyster areas. Samplings are carried out in February, June, September and December (late November in 1999). This network is a tool for trend estimations (geographic and chronological, on the national scale) ; it must not be considered as an accurate estimation of professional oyster ongrowings. Based on REMORA results, generally tallied with professional observations, and with comparison to previous years, 1999 exhibited mortality in adults (23%) and poor growth in both adults (63.0 g in late November) and...
Tipo: Text Palavras-chave: Polydora Quality Growth Mortality Crassosirea gigas Pacific cupped oyster France REMORA; Polydora Qualité Croissance Mortalité Crassosirea gigas Huître creuse France REMORA.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1634.pdf
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Application of high-throughput sequencing to population differentiation in the cupped oysters Crassostrea angulata/C. gigas ArchiMer
Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie.
The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf
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Analysis of genome-wide differentiation between native and introduced populations of the cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie.
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end...
Tipo: Text Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf
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Ressources génétiques chez l'huître creuse Crassostrea gigas : variabilité, différenciation et adaptation aux conditions de production en Charente Maritime ArchiMer
Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie.
es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)...
Tipo: Text Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf
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A comparison of growth and biochemical composition of Mytilus galloprovincialis (Lmk.) and Mytilus edulis (L.) on the West coast of Cotentin, Normandy, France 1999-2000 ArchiMer
Kopp, Joel; Cornette, Florence; Simonne, Charlotte.
The blue mussel, Mytilus edulis has been reared along the Normandy coast line since the 1960s. The gonadal cycle of this mussel species shows a sharp decrease in meat quality during the winter period after spawning. This decline in meat quality is so severe that sales have to be suspended from December to July. Another species of mussel, Mytilus galloprovincialis, which is reared in the lagoons along the French shore of the Mediterranean Sea has a different spawning cycle. An experiment was undertaken to study the meat quality of M. Galloprovincialis throughout the year after the spat were transferred from the Mediterranean Sea to the Normandy coast. This species showed an immediate adaptation to the gonadal cycle of M. edulis. Despite suggestions from...
Tipo: Text Palavras-chave: Meat quality; Mytilus galloprovincialis; Mytilus edulis; Gonadal cycle.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-422.pdf
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Etat des stocks conchylicoles normands en 2000 ArchiMer
Kopp, Joel; Messiaen, Gregory; Le Gagneur, Eric; Cornette, Florence; Ropert, Michel.
Crassostrea gigas and Mytilus edulis are reared in four areas along the eastern coast of Cotentin (Normandy). Every five years, a stock assessment survey, based on a stratified random sampling is carried out using aerial photographs. During September 2000, oysters bags and mussel banks random samplings have been weighed on leasing grounds and in random sampled farms in order to evaluate the overall total gross and net weights breeded (oysters 40 000 and mussels 17 000 metric tons, net weights). Age compositions of each stock in each rearing area have been computed. This last stock assessment showed that cultural regulations are less and less observed in Normandy's breeding areas. A strong increase in bag densities has been noticed since 1995. As stock...
Tipo: Text Palavras-chave: Ostréiculture; Mytiliculture; Estimation des stocks; Croissance; Rendements; Oysters culture; Mussels culture; Stock assessment; Growth; Yields.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00107/21786/19360.pdf
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